isotype control mouse igg1 (Revvity)
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Isotype Control Mouse Igg1, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-Mouse+IgG1+(isotyping)+AlphaLISA+Acceptor+beads/pmc09890142__mct___22___0312_supplementary_table_s1_suppst1-0-38-43
Average 93 stars, based on 143 article reviews
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Concentration Assay:Article Title: Genome-wide CRISPR screen identifies protein pathways modulating tau protein levels in neurons Article Snippet: Briefly, human recombinant Tau 441 was used for standard curve (Perkin Elmer #AL271S) prepared in a 12-point dilution curve (2-fold dilutions) diluted in 1× Immunoassay buffer (Perkin Elmer #AL00F). .. Biotinylated mouse anti-Tau monoclonal antibody HT7 (ThermoFisher Scientific #MN100B: 100 μg/ml) and a mouse anti-Tau monoclonal antibody BT2 (ThermoFisher Scientific #MN1010) was conjugated to Perkin Elmer Article Title: AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains Article Snippet: Briefly, 10 μl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was pre-incubated with either 10 μl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 μl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693). .. Subsequently, 10 μl of Protein A Article Title: Comparative Proteomic and Transcriptomic Analysis of the Impact of Androgen Stimulation and Darolutamide Inhibition. Article Snippet: The monoclonal mouse anti-human antibody M356201-2 (Dako, Glostrup, Denmark) and the polyclonal rabbit anti-human antibody 06-680 (Merck Millipore, Burlington, MA, USA) were used for AR Cancers 2023, 15, 2 4 of 17 detection, as previously described [32]. .. Incubation:Article Title: Isolation and Characterization of Urine Microvesicles from Prostate Cancer Patients: Different Approaches, Different Visions Article Snippet: Five μL of EVs resuspended in PBS were transferred to a 96-well white 1/2 area microplate (Perkin Elmer, Madrid, Spain). .. Samples were incubated overnight at 4oC with 10 μL/well of anti-human CD9 antibody (SHI-EXO-M01-50; CosmoBio Co, Tokyo, Japan) conjugated to Article Title: AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains Article Snippet: Briefly, 10 μl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was pre-incubated with either 10 μl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 μl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693). .. Subsequently, 10 μl of Protein A Comparison:Article Title: Comparative Proteomic and Transcriptomic Analysis of the Impact of Androgen Stimulation and Darolutamide Inhibition. Article Snippet: AlphaLISA donor beads conjugated to anti-mouse IgG antibody (AS104D, PerkinElmer) were used to couple to the rabbit primary antibodies at a final concentration of 40 μg/mL. .. For comparison, |
