Review



isotype control mouse igg1  (Revvity)


Bioz Verified Symbol Revvity is a verified supplier
Bioz Manufacturer Symbol Revvity manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Revvity isotype control mouse igg1
    Isotype Control Mouse Igg1, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-Mouse+IgG1+(isotyping)+AlphaLISA+Acceptor+beads/pmc09890142__mct___22___0312_supplementary_table_s1_suppst1-0-38-43
    Average 93 stars, based on 143 article reviews
    isotype control mouse igg1 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Genome-wide CRISPR screen identifies protein pathways modulating tau protein levels in neurons
    Article Snippet: Briefly, human recombinant Tau 441 was used for standard curve (Perkin Elmer #AL271S) prepared in a 12-point dilution curve (2-fold dilutions) diluted in 1× Immunoassay buffer (Perkin Elmer #AL00F). .. Biotinylated mouse anti-Tau monoclonal antibody HT7 (ThermoFisher Scientific #MN100B: 100 μg/ml) and a mouse anti-Tau monoclonal antibody BT2 (ThermoFisher Scientific #MN1010) was conjugated to Perkin Elmer AlphaLISA acceptor beads (Perkin Elmer, # 6772002), to a final 5 mg beads/ml concentration. ..

    Article Title: AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains
    Article Snippet: Briefly, 10 μl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was pre-incubated with either 10 μl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 μl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693). .. Subsequently, 10 μl of Protein A AlphaLISA ® Acceptor beads (10 μg/ml final concentration; Perkin Elmer cat# AL101C) were added, and further incubated for 1 hour at room temperature in the dark with shaking (250 rpm). .. Following this incubation, 20 μl of Streptavidin Donor beads (40 μg/ml final concentration; Perkin Elmer cat# 6760002S) were added, and the microplates were further incubated for 1 hour at room temperature in the dark with shaking (250 rpm) and read on FLUOStar ® Omega multi-mode microplate reader instrument (BMG Labtech) (excitation 680 nm, emission 615 nm).

    Article Title: Comparative Proteomic and Transcriptomic Analysis of the Impact of Androgen Stimulation and Darolutamide Inhibition.
    Article Snippet: The monoclonal mouse anti-human antibody M356201-2 (Dako, Glostrup, Denmark) and the polyclonal rabbit anti-human antibody 06-680 (Merck Millipore, Burlington, MA, USA) were used for AR Cancers 2023, 15, 2 4 of 17 detection, as previously described [32]. .. AlphaLISA acceptor beads conjugated to anti-rabbit IgG antibody (AL104, PerkinElmer) were used to couple to the mouse primary antibodies at a final concentration of 10 μg/mL. .. AlphaLISA donor beads conjugated to anti-mouse IgG antibody (AS104D, PerkinElmer) were used to couple to the rabbit primary antibodies at a final concentration of 40 μg/mL.

    Incubation:

    Article Title: Isolation and Characterization of Urine Microvesicles from Prostate Cancer Patients: Different Approaches, Different Visions
    Article Snippet: Five μL of EVs resuspended in PBS were transferred to a 96-well white 1/2 area microplate (Perkin Elmer, Madrid, Spain). .. Samples were incubated overnight at 4oC with 10 μL/well of anti-human CD9 antibody (SHI-EXO-M01-50; CosmoBio Co, Tokyo, Japan) conjugated to AlphaLisa acceptor beads (10 μg/mL; 6772001, Perkin Elmer, Madrid, Spain), and 10 μL/well of biotinylated human anti-CD63 antibody (3 nM, SHI-EXO-M02-50, CosmoBio Co, Tokyo, Japan) previously biotinylated. .. Then, 25 μL/well of AlphaScreenTM streptavidin-coated donor beads (40 μg/mL; 6760002, Perkin Elmer, Madrid, Spain) were added and incubated in the dark for another 30 min at RT.

    Article Title: AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains
    Article Snippet: Briefly, 10 μl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was pre-incubated with either 10 μl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 μl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693). .. Subsequently, 10 μl of Protein A AlphaLISA ® Acceptor beads (10 μg/ml final concentration; Perkin Elmer cat# AL101C) were added, and further incubated for 1 hour at room temperature in the dark with shaking (250 rpm). .. Following this incubation, 20 μl of Streptavidin Donor beads (40 μg/ml final concentration; Perkin Elmer cat# 6760002S) were added, and the microplates were further incubated for 1 hour at room temperature in the dark with shaking (250 rpm) and read on FLUOStar ® Omega multi-mode microplate reader instrument (BMG Labtech) (excitation 680 nm, emission 615 nm).

    Comparison:

    Article Title: Comparative Proteomic and Transcriptomic Analysis of the Impact of Androgen Stimulation and Darolutamide Inhibition.
    Article Snippet: AlphaLISA donor beads conjugated to anti-mouse IgG antibody (AS104D, PerkinElmer) were used to couple to the rabbit primary antibodies at a final concentration of 40 μg/mL. .. For comparison, AlphaLISA acceptor beads conjugated to anti-mouse IgG antibody (AL105C, PerkinElmer) and AlphaLISA donor beads conjugated to anti-rabbit IgG antibody (AS105M, PerkinElmer) were used. .. Signals were measured after overnight incubation using 615 nm luminescence emission and an Envision Alpha plate reader (PerkinElmer).



    Similar Products

    93
    Revvity isotype control mouse igg1
    Isotype Control Mouse Igg1, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-Mouse+IgG1+(isotyping)+AlphaLISA+Acceptor+beads/pmc09890142__mct___22___0312_supplementary_table_s1_suppst1-0-38-43
    Average 93 stars, based on 1 article reviews
    isotype control mouse igg1 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Novo Nordisk alphalisa® acceptor beads pre-conjugated with nn454-1f31
    Alphalisa® Acceptor Beads Pre Conjugated With Nn454 1f31, supplied by Novo Nordisk, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/alphalisa++acceptor+beads+pre+conjugated+with+nn454+1f31/pmc11634773__41586_2024_8202_MOESM1_ESM-221-13-14
    Average 90 stars, based on 1 article reviews
    alphalisa® acceptor beads pre-conjugated with nn454-1f31 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Bethyl erns protein-conjugated alphalisa acceptor beads
    Erns Protein Conjugated Alphalisa Acceptor Beads, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/erns+protein+conjugated+alphalisa+acceptor+beads/pm38136839-105-30-43
    Average 90 stars, based on 1 article reviews
    erns protein-conjugated alphalisa acceptor beads - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    91
    Revvity mouse igg2a
    Mouse Igg2a, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-Mouse+IgG2a+(isotyping)+AlphaLISA+Acceptor+beads/pmc10373297-192-48-54
    Average 91 stars, based on 1 article reviews
    mouse igg2a - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    91
    Revvity isotype control igg2a
    Isotype Control Igg2a, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-Mouse+IgG2a+(isotyping)+AlphaLISA+Acceptor+beads/pmc10486495-53-80-83
    Average 91 stars, based on 1 article reviews
    isotype control igg2a - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    91
    Revvity isotypic rat igg
    Isotypic Rat Igg, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-rat+IgG+(Fc+specific)+AlphaLISA+Acceptor+Beads/pmc10433716-54-0-3
    Average 91 stars, based on 1 article reviews
    isotypic rat igg - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    91
    Revvity mouse igg2b
    Mouse Igg2b, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-Mouse+IgG2b+(isotyping)+AlphaLISA+Acceptor+beads/pmc10465578-321-8-14
    Average 91 stars, based on 1 article reviews
    mouse igg2b - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    91
    Revvity alphalisa anti flag acceptor
    Alphalisa Anti Flag Acceptor, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-FLAG+AlphaLISA+Acceptor+Beads/us11739085-1591-7-10
    Average 91 stars, based on 1 article reviews
    alphalisa anti flag acceptor - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    93
    Revvity mouse igg1
    Representative images ( a ) and quantification ( b ) of βIII-tubulin + neuronal axon (white arrows) length following equal amount (1 μg) of WT and HepaCAM-depleted A-Exo. treatment. i control; ii WT A-Exo.; iii HepaCAM KO A-Exo.; HepaCAM-depleted A-Exo. were prepared from HepaCAM KO astrocyte cultures as described in materials and methods. Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (5–8 neurons/replicate, >3 biological replicates)/group; Representative images ( c ) and quantification ( d ) of βIII-tubulin + neuronal axon (white arrows) length following co-treatment with HepaCAM antibody (ProteinTech) and A-Exo. i control (1 x PBS); ii <t>IgG</t> alone; iii A-Exo. + IgG; iv A-Exo. + HepaCAM ab; 8 μg ab/coverslip (12 mm diameter) was used in the treatment. Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (7–9 neurons/replicate, ≥2 biological replicates)/group; Representative images ( e ) and quantification ( f ) of βIII-tubulin + neuronal axon (white arrows) length following HepaCAM ECD coating. i PDL alone; ii PDL + BSA (4 μg); iii PDL + HepaCAM ECD (4 μg); Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (8–12 neurons/replicate, ≥3 biological replicates)/group; 1 μg A-Exo. was used in each experiment; p values in ( b , d , f ) determined using one-way ANOVA followed by a Tukey post hoc test. Data are presented as mean values ± SEM.
    Mouse Igg1, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+acceptor+beads/Anti-Mouse+IgG1+(isotyping)+AlphaLISA+Acceptor+beads/pmc10449881-377-20-24
    Average 93 stars, based on 1 article reviews
    mouse igg1 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Representative images ( a ) and quantification ( b ) of βIII-tubulin + neuronal axon (white arrows) length following equal amount (1 μg) of WT and HepaCAM-depleted A-Exo. treatment. i control; ii WT A-Exo.; iii HepaCAM KO A-Exo.; HepaCAM-depleted A-Exo. were prepared from HepaCAM KO astrocyte cultures as described in materials and methods. Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (5–8 neurons/replicate, >3 biological replicates)/group; Representative images ( c ) and quantification ( d ) of βIII-tubulin + neuronal axon (white arrows) length following co-treatment with HepaCAM antibody (ProteinTech) and A-Exo. i control (1 x PBS); ii IgG alone; iii A-Exo. + IgG; iv A-Exo. + HepaCAM ab; 8 μg ab/coverslip (12 mm diameter) was used in the treatment. Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (7–9 neurons/replicate, ≥2 biological replicates)/group; Representative images ( e ) and quantification ( f ) of βIII-tubulin + neuronal axon (white arrows) length following HepaCAM ECD coating. i PDL alone; ii PDL + BSA (4 μg); iii PDL + HepaCAM ECD (4 μg); Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (8–12 neurons/replicate, ≥3 biological replicates)/group; 1 μg A-Exo. was used in each experiment; p values in ( b , d , f ) determined using one-way ANOVA followed by a Tukey post hoc test. Data are presented as mean values ± SEM.

    Journal: Nature Communications

    Article Title: Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation

    doi: 10.1038/s41467-023-40926-2

    Figure Lengend Snippet: Representative images ( a ) and quantification ( b ) of βIII-tubulin + neuronal axon (white arrows) length following equal amount (1 μg) of WT and HepaCAM-depleted A-Exo. treatment. i control; ii WT A-Exo.; iii HepaCAM KO A-Exo.; HepaCAM-depleted A-Exo. were prepared from HepaCAM KO astrocyte cultures as described in materials and methods. Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (5–8 neurons/replicate, >3 biological replicates)/group; Representative images ( c ) and quantification ( d ) of βIII-tubulin + neuronal axon (white arrows) length following co-treatment with HepaCAM antibody (ProteinTech) and A-Exo. i control (1 x PBS); ii IgG alone; iii A-Exo. + IgG; iv A-Exo. + HepaCAM ab; 8 μg ab/coverslip (12 mm diameter) was used in the treatment. Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (7–9 neurons/replicate, ≥2 biological replicates)/group; Representative images ( e ) and quantification ( f ) of βIII-tubulin + neuronal axon (white arrows) length following HepaCAM ECD coating. i PDL alone; ii PDL + BSA (4 μg); iii PDL + HepaCAM ECD (4 μg); Scale bar: 100 μm; Number of neurons quantified in each group shown in the graph (8–12 neurons/replicate, ≥3 biological replicates)/group; 1 μg A-Exo. was used in each experiment; p values in ( b , d , f ) determined using one-way ANOVA followed by a Tukey post hoc test. Data are presented as mean values ± SEM.

    Article Snippet: For immunoprecipitation, Dynabeads® M-270 Epoxy beads (Thermo Fisher Scientific) with anti-CD81 (clone Eat-2, BioLegend), anti-HepaCAM (Affinity Biosciences, # DF12075), and mouse IgG1 (clone MG1-45 BioLegend) was conjugated individually according to the instructions of the Dynabeads Antibody Coupling Kit (Thermo Fisher Scientific).

    Techniques: Control